GrippeEspagneV1, Main, Exploration, bibRecord, 000180

Evaluation of a new, rapid, simple test for the detection of influenza virus.

Identifieur interne : 000180 ( Main/Exploration ); précédent : 000179; suivant : 000181

Evaluation of a new, rapid, simple test for the detection of influenza virus.

Auteurs : Juan Carlos Hurtado [Espagne] ; Maria Mar Mosquera [Espagne] ; Elisa De Lazzari [Espagne] ; Esteban Martínez [Espagne] ; Nuria Torner [Espagne] ; Ricard Isanta [Espagne] ; Patricia De Molina [Espagne] ; Tomás Pumarola [Espagne] ; Maria Angeles Marcos [Espagne] ; Jordi Vila ; Jordi Vila Estape

Source :

BMC infectious diseases [ 1471-2334 ] ; 2015.

RBID : pubmed:25656393

Descripteurs français

KwdFr :
- ARN viral (génétique), ARN viral (isolement et purification), Adulte (MeSH), Enfant (MeSH), Espagne (épidémiologie), Facteurs temps (MeSH), Grippe humaine (diagnostic), Grippe humaine (virologie), Grippe humaine (épidémiologie), Humains (MeSH), Nez (virologie), Pharynx (virologie), Réaction de polymérisation en chaine en temps réel (MeSH), Sensibilité et spécificité (MeSH), Techniques d'amplification d'acides nucléiques (méthodes), Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification), Virus influenza B (génétique), Virus influenza B (isolement et purification).
MESH :
- diagnostic : Grippe humaine.
- génétique : ARN viral, Virus de la grippe A, Virus influenza B.
- isolement et purification : ARN viral, Virus de la grippe A, Virus influenza B.
- méthodes : Techniques d'amplification d'acides nucléiques.
- virologie : Grippe humaine, Nez, Pharynx.
- épidémiologie : Espagne, Grippe humaine.
- Adulte, Enfant, Facteurs temps, Humains, Réaction de polymérisation en chaine en temps réel, Sensibilité et spécificité.
Wicri :
- geographic : Espagne.

English descriptors

KwdEn :
- Adult (MeSH), Child (MeSH), Humans (MeSH), Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza B virus (genetics), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (epidemiology), Influenza, Human (virology), Nose (virology), Nucleic Acid Amplification Techniques (methods), Pharynx (virology), RNA, Viral (genetics), RNA, Viral (isolation & purification), Real-Time Polymerase Chain Reaction (MeSH), Sensitivity and Specificity (MeSH), Spain (epidemiology), Time Factors (MeSH).
MESH :
- chemical , genetics : RNA, Viral.
- geographic , epidemiology : Spain.
- diagnosis : Influenza, Human.
- epidemiology : Influenza, Human.
- genetics : Influenza A virus, Influenza B virus.
- isolation & purification : Influenza A virus, Influenza B virus, RNA, Viral.
- methods : Nucleic Acid Amplification Techniques.
- virology : Influenza, Human, Nose, Pharynx.
- Adult, Child, Humans, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity, Time Factors.

Abstract

BACKGROUND

Influenza virus infections are responsible for significant morbidity and mortality in both pediatric and adult populations worldwide. Rapid and accurate diagnosis of influenza is necessary for appropriate patient management during the influenza season and for optimal utilization of anti-influenza therapy. We prospectively tested the accuracy of a simple and rapid diagnostic method.

METHODS

Ninety-eight samples (nasal and pharyngeal swabs) from patients with upper respiratory tract infection symptoms who presented to primary healthcare centres in Barcelona (Spain) were prospectively analyzed. The samples were collected as part of influenza surveillance program. Samples that had enough volume to make the new test after aliquoting the amount needed to perform routine tests were included. None of the samples were pre-selected as a result of their status in relation to influenza virus. Samples were analyzed by in-house real-time PCR and Alere i Influenza A & B (Alere i), which uses isothermal amplification of nucleic acids for the qualitative detection of influenza A and B in nasal swabs transported in viral transport media. The two techniques were compared by positive percent agreement (PPA) and negative percent agreement (NPA). Statistical analysis was performed with Stata.

RESULTS

Of the 98 samples analysed 90 were concordant; 46 (46.9%) were positive and 44 (44.9%) were negative. Five samples showed invalid results with the Alere i test and could be not re-tested due to insufficient sample volume and were not included in the final statistical analysis. In the 93 remaining samples, the Alere i test showed 97% of accuracy having correctly classified 90 samples. We obtained discordant results in 3 samples (3%). The PPA was 93.8% for influenza A and 94.1% for influenza B, and NPA was 100% for influenza A and influenza B virus. In addition, the Alere i was very rapid (15 minutes or less) and extremely easy to use.

CONCLUSIONS

The Alere i test provided a good correlation compared to the real-time PCR test for the diagnosis of influenza. Since this method can be performed in minutes, it allows immediate, accurate clinical decisions to prescribe appropriate antiviral treatment or isolation of patients.

DOI: 10.1186/s12879-015-0775-5
PubMed: 25656393
PubMed Central: PMC4369360

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 00183
to stream Main, to step Curation: 000183

Le document en format XML

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<author><name sortKey="Marcos, Maria Angeles" sort="Marcos, Maria Angeles" uniqKey="Marcos M" first="Maria Angeles" last="Marcos">Maria Angeles Marcos</name>
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<author><name sortKey="Estape, Jordi Vila" sort="Estape, Jordi Vila" uniqKey="Estape J" first="Jordi Vila" last="Estape">Jordi Vila Estape</name>
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<wicri:regionArea>Barcelona Centre for International Health Research (CRESIB, Hospital Clinic-Universitat de Barcelona), Barcelona</wicri:regionArea>
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<author><name sortKey="Martinez, Esteban" sort="Martinez, Esteban" uniqKey="Martinez E" first="Esteban" last="Martínez">Esteban Martínez</name>
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<wicri:regionArea>Infectious Diseases Unit, Hospital Clinic-IDIBAPS, University of Barcelona, Barcelona</wicri:regionArea>
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<author><name sortKey="Torner, Nuria" sort="Torner, Nuria" uniqKey="Torner N" first="Nuria" last="Torner">Nuria Torner</name>
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<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Public Health Agency of Catalonia, Barcelona</wicri:regionArea>
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<affiliation wicri:level="3"><nlm:affiliation>CIBER Epidemiology and Public Health (CIBERESP), Madrid, Spain. nuria.torner@gencat.cat.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>CIBER Epidemiology and Public Health (CIBERESP), Madrid</wicri:regionArea>
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<author><name sortKey="Isanta, Ricard" sort="Isanta, Ricard" uniqKey="Isanta R" first="Ricard" last="Isanta">Ricard Isanta</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain. risanta@clinic.ub.es.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona</wicri:regionArea>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
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<author><name sortKey="De Molina, Patricia" sort="De Molina, Patricia" uniqKey="De Molina P" first="Patricia" last="De Molina">Patricia De Molina</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain. patriciademolina@gmail.com.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona</wicri:regionArea>
<placeName><settlement type="city">Barcelone</settlement>
<region nuts="2" type="region">Catalogne</region>
</placeName>
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<author><name sortKey="Pumarola, Tomas" sort="Pumarola, Tomas" uniqKey="Pumarola T" first="Tomás" last="Pumarola">Tomás Pumarola</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Microbiology, Hospital Universitari Vall d'Hebron (HUVH), Universidad Autónoma de Barcelona (UAB), Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. tpumarola@vhebron.net.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Microbiology, Hospital Universitari Vall d'Hebron (HUVH), Universidad Autónoma de Barcelona (UAB), Vall d'Hebron Institut de Recerca (VHIR), Barcelona</wicri:regionArea>
<placeName><settlement type="city">Barcelone</settlement>
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<author><name sortKey="Marcos, Maria Angeles" sort="Marcos, Maria Angeles" uniqKey="Marcos M" first="Maria Angeles" last="Marcos">Maria Angeles Marcos</name>
<affiliation wicri:level="3"><nlm:affiliation>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain. mmarcos@clinic.ub.es.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona</wicri:regionArea>
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<region nuts="2" type="region">Catalogne</region>
</placeName>
</affiliation>
<affiliation wicri:level="3"><nlm:affiliation>Barcelona Centre for International Health Research (CRESIB, Hospital Clinic-Universitat de Barcelona), Barcelona, Spain. mmarcos@clinic.ub.es.</nlm:affiliation>
<country xml:lang="fr">Espagne</country>
<wicri:regionArea>Barcelona Centre for International Health Research (CRESIB, Hospital Clinic-Universitat de Barcelona), Barcelona</wicri:regionArea>
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<author><name sortKey="Vila, Jordi" sort="Vila, Jordi" uniqKey="Vila J" first="Jordi" last="Vila">Jordi Vila</name>
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<author><name sortKey="Estape, Jordi Vila" sort="Estape, Jordi Vila" uniqKey="Estape J" first="Jordi Vila" last="Estape">Jordi Vila Estape</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term>
<term>Child (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Influenza A virus (genetics)</term>
<term>Influenza A virus (isolation & purification)</term>
<term>Influenza B virus (genetics)</term>
<term>Influenza B virus (isolation & purification)</term>
<term>Influenza, Human (diagnosis)</term>
<term>Influenza, Human (epidemiology)</term>
<term>Influenza, Human (virology)</term>
<term>Nose (virology)</term>
<term>Nucleic Acid Amplification Techniques (methods)</term>
<term>Pharynx (virology)</term>
<term>RNA, Viral (genetics)</term>
<term>RNA, Viral (isolation & purification)</term>
<term>Real-Time Polymerase Chain Reaction (MeSH)</term>
<term>Sensitivity and Specificity (MeSH)</term>
<term>Spain (epidemiology)</term>
<term>Time Factors (MeSH)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term>
<term>ARN viral (isolement et purification)</term>
<term>Adulte (MeSH)</term>
<term>Enfant (MeSH)</term>
<term>Espagne (épidémiologie)</term>
<term>Facteurs temps (MeSH)</term>
<term>Grippe humaine (diagnostic)</term>
<term>Grippe humaine (virologie)</term>
<term>Grippe humaine (épidémiologie)</term>
<term>Humains (MeSH)</term>
<term>Nez (virologie)</term>
<term>Pharynx (virologie)</term>
<term>Réaction de polymérisation en chaine en temps réel (MeSH)</term>
<term>Sensibilité et spécificité (MeSH)</term>
<term>Techniques d'amplification d'acides nucléiques (méthodes)</term>
<term>Virus de la grippe A (génétique)</term>
<term>Virus de la grippe A (isolement et purification)</term>
<term>Virus influenza B (génétique)</term>
<term>Virus influenza B (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>Spain</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Influenza, Human</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Influenza A virus</term>
<term>Influenza B virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term>
<term>Virus de la grippe A</term>
<term>Virus influenza B</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term>
<term>Influenza B virus</term>
<term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term>
<term>Virus de la grippe A</term>
<term>Virus influenza B</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Nucleic Acid Amplification Techniques</term>
</keywords>
<keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Techniques d'amplification d'acides nucléiques</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term>
<term>Nez</term>
<term>Pharynx</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Influenza, Human</term>
<term>Nose</term>
<term>Pharynx</term>
</keywords>
<keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Espagne</term>
<term>Grippe humaine</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Adult</term>
<term>Child</term>
<term>Humans</term>
<term>Real-Time Polymerase Chain Reaction</term>
<term>Sensitivity and Specificity</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Adulte</term>
<term>Enfant</term>
<term>Facteurs temps</term>
<term>Humains</term>
<term>Réaction de polymérisation en chaine en temps réel</term>
<term>Sensibilité et spécificité</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Espagne</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Influenza virus infections are responsible for significant morbidity and mortality in both pediatric and adult populations worldwide. Rapid and accurate diagnosis of influenza is necessary for appropriate patient management during the influenza season and for optimal utilization of anti-influenza therapy. We prospectively tested the accuracy of a simple and rapid diagnostic method.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>Ninety-eight samples (nasal and pharyngeal swabs) from patients with upper respiratory tract infection symptoms who presented to primary healthcare centres in Barcelona (Spain) were prospectively analyzed. The samples were collected as part of influenza surveillance program. Samples that had enough volume to make the new test after aliquoting the amount needed to perform routine tests were included. None of the samples were pre-selected as a result of their status in relation to influenza virus. Samples were analyzed by in-house real-time PCR and Alere i Influenza A & B (Alere i), which uses isothermal amplification of nucleic acids for the qualitative detection of influenza A and B in nasal swabs transported in viral transport media. The two techniques were compared by positive percent agreement (PPA) and negative percent agreement (NPA). Statistical analysis was performed with Stata.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Of the 98 samples analysed 90 were concordant; 46 (46.9%) were positive and 44 (44.9%) were negative. Five samples showed invalid results with the Alere i test and could be not re-tested due to insufficient sample volume and were not included in the final statistical analysis. In the 93 remaining samples, the Alere i test showed 97% of accuracy having correctly classified 90 samples. We obtained discordant results in 3 samples (3%). The PPA was 93.8% for influenza A and 94.1% for influenza B, and NPA was 100% for influenza A and influenza B virus. In addition, the Alere i was very rapid (15 minutes or less) and extremely easy to use.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b>
</p>
<p>The Alere i test provided a good correlation compared to the real-time PCR test for the diagnosis of influenza. Since this method can be performed in minutes, it allows immediate, accurate clinical decisions to prescribe appropriate antiviral treatment or isolation of patients.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">25656393</PMID>
<DateCompleted><Year>2015</Year>
<Month>12</Month>
<Day>18</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2334</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>15</Volume>
<PubDate><Year>2015</Year>
<Month>Feb</Month>
<Day>06</Day>
</PubDate>
</JournalIssue>
<Title>BMC infectious diseases</Title>
<ISOAbbreviation>BMC Infect. Dis.</ISOAbbreviation>
</Journal>
<ArticleTitle>Evaluation of a new, rapid, simple test for the detection of influenza virus.</ArticleTitle>
<Pagination><MedlinePgn>44</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s12879-015-0775-5</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Influenza virus infections are responsible for significant morbidity and mortality in both pediatric and adult populations worldwide. Rapid and accurate diagnosis of influenza is necessary for appropriate patient management during the influenza season and for optimal utilization of anti-influenza therapy. We prospectively tested the accuracy of a simple and rapid diagnostic method.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">Ninety-eight samples (nasal and pharyngeal swabs) from patients with upper respiratory tract infection symptoms who presented to primary healthcare centres in Barcelona (Spain) were prospectively analyzed. The samples were collected as part of influenza surveillance program. Samples that had enough volume to make the new test after aliquoting the amount needed to perform routine tests were included. None of the samples were pre-selected as a result of their status in relation to influenza virus. Samples were analyzed by in-house real-time PCR and Alere i Influenza A & B (Alere i), which uses isothermal amplification of nucleic acids for the qualitative detection of influenza A and B in nasal swabs transported in viral transport media. The two techniques were compared by positive percent agreement (PPA) and negative percent agreement (NPA). Statistical analysis was performed with Stata.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Of the 98 samples analysed 90 were concordant; 46 (46.9%) were positive and 44 (44.9%) were negative. Five samples showed invalid results with the Alere i test and could be not re-tested due to insufficient sample volume and were not included in the final statistical analysis. In the 93 remaining samples, the Alere i test showed 97% of accuracy having correctly classified 90 samples. We obtained discordant results in 3 samples (3%). The PPA was 93.8% for influenza A and 94.1% for influenza B, and NPA was 100% for influenza A and influenza B virus. In addition, the Alere i was very rapid (15 minutes or less) and extremely easy to use.</AbstractText>
<AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">The Alere i test provided a good correlation compared to the real-time PCR test for the diagnosis of influenza. Since this method can be performed in minutes, it allows immediate, accurate clinical decisions to prescribe appropriate antiviral treatment or isolation of patients.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hurtado</LastName>
<ForeName>Juan Carlos</ForeName>
<Initials>JC</Initials>
<AffiliationInfo><Affiliation>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain. jhurtado@clinic.ub.es.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mosquera</LastName>
<ForeName>Maria Mar</ForeName>
<Initials>MM</Initials>
<AffiliationInfo><Affiliation>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain. mdmosquera@clinic.ub.es.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Barcelona Centre for International Health Research (CRESIB, Hospital Clinic-Universitat de Barcelona), Barcelona, Spain. mdmosquera@clinic.ub.es.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>de Lazzari</LastName>
<ForeName>Elisa</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Barcelona Centre for International Health Research (CRESIB, Hospital Clinic-Universitat de Barcelona), Barcelona, Spain. elazzari@clinic.ub.es.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Martínez</LastName>
<ForeName>Esteban</ForeName>
<Initials>E</Initials>
<AffiliationInfo><Affiliation>Infectious Diseases Unit, Hospital Clinic-IDIBAPS, University of Barcelona, Barcelona, Spain. estebanm@clinic.ub.es.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Torner</LastName>
<ForeName>Nuria</ForeName>
<Initials>N</Initials>
<AffiliationInfo><Affiliation>Public Health Agency of Catalonia, Barcelona, Spain. nuria.torner@gencat.cat.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>CIBER Epidemiology and Public Health (CIBERESP), Madrid, Spain. nuria.torner@gencat.cat.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Isanta</LastName>
<ForeName>Ricard</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain. risanta@clinic.ub.es.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>de Molina</LastName>
<ForeName>Patricia</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain. patriciademolina@gmail.com.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Pumarola</LastName>
<ForeName>Tomás</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>Department of Microbiology, Hospital Universitari Vall d'Hebron (HUVH), Universidad Autónoma de Barcelona (UAB), Vall d'Hebron Institut de Recerca (VHIR), Barcelona, Spain. tpumarola@vhebron.net.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Marcos</LastName>
<ForeName>Maria Angeles</ForeName>
<Initials>MA</Initials>
<AffiliationInfo><Affiliation>Department of Clinical Microbiology, Hospital Clinic, School of Medicine, University of Barcelona, Barcelona, Spain. mmarcos@clinic.ub.es.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Barcelona Centre for International Health Research (CRESIB, Hospital Clinic-Universitat de Barcelona), Barcelona, Spain. mmarcos@clinic.ub.es.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Vila</LastName>
<ForeName>Jordi</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="N"><LastName>Estape</LastName>
<ForeName>Jordi Vila</ForeName>
<Initials>JV</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2015</Year>
<Month>02</Month>
<Day>06</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>BMC Infect Dis</MedlineTA>
<NlmUniqueID>100968551</NlmUniqueID>
<ISSNLinking>1471-2334</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<CommentsCorrectionsList><CommentsCorrections RefType="ErratumIn"><RefSource>BMC Infect Dis. 2015;15:383</RefSource>
<PMID Version="1">26395348</PMID>
<Note>Estape, Jordi Vila [corrected to Vila, Jordi]</Note>
</CommentsCorrections>
</CommentsCorrectionsList>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009981" MajorTopicYN="N">Influenza B virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009666" MajorTopicYN="N">Nose</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D021141" MajorTopicYN="N">Nucleic Acid Amplification Techniques</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010614" MajorTopicYN="N">Pharynx</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060888" MajorTopicYN="N">Real-Time Polymerase Chain Reaction</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013030" MajorTopicYN="N" Type="Geographic">Spain</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2014</Year>
<Month>08</Month>
<Day>18</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2015</Year>
<Month>01</Month>
<Day>20</Day>
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<ArticleId IdType="pmc">PMC4369360</ArticleId>
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<affiliations><list><country><li>Espagne</li>
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<region><li>Catalogne</li>
<li>Communauté de Madrid</li>
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<li>Madrid</li>
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<tree><noCountry><name sortKey="Estape, Jordi Vila" sort="Estape, Jordi Vila" uniqKey="Estape J" first="Jordi Vila" last="Estape">Jordi Vila Estape</name>
<name sortKey="Vila, Jordi" sort="Vila, Jordi" uniqKey="Vila J" first="Jordi" last="Vila">Jordi Vila</name>
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<country name="Espagne"><region name="Catalogne"><name sortKey="Hurtado, Juan Carlos" sort="Hurtado, Juan Carlos" uniqKey="Hurtado J" first="Juan Carlos" last="Hurtado">Juan Carlos Hurtado</name>
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<name sortKey="De Lazzari, Elisa" sort="De Lazzari, Elisa" uniqKey="De Lazzari E" first="Elisa" last="De Lazzari">Elisa De Lazzari</name>
<name sortKey="De Molina, Patricia" sort="De Molina, Patricia" uniqKey="De Molina P" first="Patricia" last="De Molina">Patricia De Molina</name>
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<name sortKey="Marcos, Maria Angeles" sort="Marcos, Maria Angeles" uniqKey="Marcos M" first="Maria Angeles" last="Marcos">Maria Angeles Marcos</name>
<name sortKey="Marcos, Maria Angeles" sort="Marcos, Maria Angeles" uniqKey="Marcos M" first="Maria Angeles" last="Marcos">Maria Angeles Marcos</name>
<name sortKey="Martinez, Esteban" sort="Martinez, Esteban" uniqKey="Martinez E" first="Esteban" last="Martínez">Esteban Martínez</name>
<name sortKey="Mosquera, Maria Mar" sort="Mosquera, Maria Mar" uniqKey="Mosquera M" first="Maria Mar" last="Mosquera">Maria Mar Mosquera</name>
<name sortKey="Mosquera, Maria Mar" sort="Mosquera, Maria Mar" uniqKey="Mosquera M" first="Maria Mar" last="Mosquera">Maria Mar Mosquera</name>
<name sortKey="Pumarola, Tomas" sort="Pumarola, Tomas" uniqKey="Pumarola T" first="Tomás" last="Pumarola">Tomás Pumarola</name>
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Evaluation of a new, rapid, simple test for the detection of influenza virus.

Evaluation of a new, rapid, simple test for the detection of influenza virus.

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